Carbonate Buffer Calculator
Phosphate Buffer Calculator

Dear researchers, we know you must have lots of work to do for your research. Considering about it, there is a sweet guy in my company developing this buffer calculator online so that you have no worries on buffer calculating. It can save your time on the process of preparing experiment. The buffer calculator can calculate various buffers which used to do experiment, including PBS Buffer, Acetic Acid-Sodium Acetate Buffer, Barbitone Sodium-HCl Buffer, Barbiturate Buffer, Borax-NaOH Buffer, Phosphate Buffer, Barbiturate Buffer, Glycine-HCl Buffer, Tris-HCl Buffer, KH₂PO₄-NaOH Buffer, et al.

Chemical/biochemical pocket companion designed by the editors of Current Protocols. Choose from a list of all of the commonly used biological buffer systems, enter your desired volume, pH, and buffer concentration, as well as the concentrations of your stock solutions of the conjugate acid and base, and the calculator will inform you of the exact volumes of these stock solutions to mix in. Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. Commonly used for various immunoassay applications and for many protein and antibody conjugation procedures, including sandwich ELISA, which require experimental surface coatings. Carbonate-bicarbonate buffer is used extensively in molecular and cell. Electrochemical energy research. Skip to content. Home; Research; Publications; Facilities; Tools. Buffer Calculator. Acetate Buffer Calculator A solution which has a stable pH is termed as a buffer solution. It is a mixture of weak acid and the base formed by the weak acid when it donates one proton. A buffer solution formed by the mixture of acetic acid and sodium acetate (base formed by acetic acid) is acetate buffer.

Even though most products pages of antibodies, proteins or other reagents will list the formula, changes are everywhere. You always need a tool to deal with them.

In addition, we have launched three research tools in succession, involving reconstitution/ molarity/dilution calculator, molecular weight calculator and ELISA data analysis.

You really should have a try. Any suggestions are warmly welcome.

Here are some common buffers you may use for your experiments.

This tool has helped you make litres of buffer (target 1,000,000 litres by September 2018)

Carbonate Buffer Calculator

The main purpose of the site is to give access to the software written for the design of thermodynamically corrected buffers. The software-

Makes corrections for the temperature at which the buffer will be used
Makes Debye-Huckel corrections for the effect of ionic strength on pKa
Describes two ways (titration or by accurate weight) for preparation of the recipe

Choose the buffer species you want to use, and enter parameters for volume, pH, and concentration of buffer species. Then, include the option to modify the ionic strength by addition of neutral salt. Finally, enter the temperature at which you'll use the buffer, and the temperature at which you'll make it up (these are often not the same). The 'Recipe’ button with then open a new window with a recipe, that you can print, or if you wish, copy the information to a lab notebook.

Phosphate Buffer Calculator